Fusion of the SRDX motif to OsPIL11 or OsPIL16 causes rice constitutively photomorphogenic phenotypes in darkness

Dark-grown seedlings develop skotomorphogenesis. Because of the development rice direct seeding cultivation systems, there is an increasing need for clarifying molecular mechanism underlying skotomorphogenic development. It has been reported that SRDX motif, LDLDLELRLGFA, was able to convert a transcriptional activator into strong repressor. In present study, explore functions PILs in skotomorphogenesis, we generated OsPIL11-SRDX and OsPIL16-SRDX transgenic lines by fusing repressor motif C-terminal two members phytochrome interacting factor-like (OsPIL) family (OsPIL11 OsPIL16). The grown darkness had constitutively photomorphogenic phenotypes with short coleoptiles open leaf blades. results RNA sequencing analysis revealed dark-grown gene expression patterns similar those wild-type under red light. Kyoto Encyclopedia Genes Genomes pathway enrichment analyses indicated levels genes related photosynthesis, photosynthesis–antenna proteins, porphyrin chlorophyll metabolism were up-regulated lines, whereas auxin down-regulated. contrast, these photosynthesis-related down-regulated overexpressing OsPIL11 or OsPIL16, which exaggerated Considered together, our data indicate OsPIL16 primarily function as activators, at least regards promoting skotomorphogenesis repressing genes.